Title: Working protocol for isolation and identification of bioactive compounds from aqueous extract of medicinal plants

Authors: Sundus Tewfik

Addresses: Department of Health and Human Sciences, London Metropolitan University, 166-220 Holloway Road, London N7 8DB, UK

Abstract: Considerable efforts have been directed towards the isolation and identification of compounds from medicinal plants, which are most likely to be responsible for the reported bioactivities. This article aimed to illustrate rationale steps involved to characterise chemical constituents and biological activities of aqueous extract of Medicinal Plant Materials (MPM). The elected, sequenced, optimised analytical techniques and bioassays have successfully unfolded potential bioactive ingredients in roots of Anacyclus pyrethrum. These chemical ingredients are responsible for the effects exerted by such medicinal plant. This presented working protocol proved to be valid and suitable for a wider range of medicinal plants research investigation.

Keywords: bioactive compounds; isolation; identification; medicinal plants; aqueous extract; chemical constituents; biological activities; authentication; herbal drugs; herbal medicines.

DOI: 10.1504/WRSTSD.2007.012662

World Review of Science, Technology and Sustainable Development, 2007 Vol.4 No.1, pp.86 - 97

Published online: 05 Mar 2007 *

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