Title: Obtaining pesticides and fertilisers for hyper production of citric acid application in smart agriculture system

Authors: S.A. Saduakhasova; B.S. Shaimenova; Y.Y. Yermekov; L.A. Murat; V.S. Kiyan; G.K. Ospankulova

Addresses: S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan ' S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan ' S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan ' S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan ' S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan ' S. Seifullin Kazakh Agro Technical University NCJSC, 010011, Nur-Sultan Zhenis Avenue, 62, Republic of Kazakhstan

Abstract: Fertilisers and pesticides play an important role in smart agriculture system where the ancient chemical fertilisers are replaced with micro fertilisers. This study investigates properties of natural and screened mutated strains exposed to X-ray irradiation and N-nitroso-N-methylurea (NMU), of Aspergillus niger. Studies have shown the specificity of natural strains to various carbon sources, which is possibly associated with a difference in the set and activity of enzymes present in the cells. An analysis of the results showed that after exposure to 1% NMU the resultant mutant strains had an increase in acid production increased by 1.2-1.4 times after the first stage, and 1.4-1.5 times after the second-stage of chemical mutagenesis by X-ray irradiation with a dose of 5.4 microsievert of the parent strain A. nigerCA7 led to an increase in acid-forming ability by 1.9 times, and the combined mutagenesis of the mutant strain A. nigerCA7-11(2)-312 increased it by 2.4 times. Studies have shown the effectiveness of combined mutagenesis, in comparison with the impact of separate chemical or physical mutagens on microorganisms.

Keywords: microorganism breeding; mutation; citric acid; Aspergillus niger; smart agriculture.

DOI: 10.1504/IJARGE.2021.10045098

International Journal of Agricultural Resources, Governance and Ecology, 2021 Vol.17 No.2/3/4, pp.271 - 286

Received: 06 Mar 2021
Accepted: 07 Jun 2021

Published online: 23 Mar 2022 *

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