Title: Preparation of porous core shell copper zinc oxide nanospheres for lysozyme delivery

Authors: Yusilawati Ahmad Nor; Farahanim Johari; Anand Kumar Meka; Hao Song; Dzun Noraini Jimat; Nur Ayuni Jamal

Addresses: Department of Biotechnology Engineering, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia ' Department of Biotechnology Engineering, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia ' Pharmacy Australia Centre of Excellent, The University of Queensland, St. Lucia QLD 4072, Australia ' Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St. Lucia QLD 4072, Australia ' Department of Biotechnology Engineering, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia ' Department of Material and Manufacturing Engineering, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia

Abstract: Metal oxide nanoparticles such as copper oxide (CuO) and zinc oxide (ZnO) have been widely used in various applications including drug delivery. Recently, there has been great progress in the development of these metal oxide nanoparticles as carrier for the delivery of antimicrobial biomolecules such as lysozyme. However, very limited amount of biomolecules can be loaded on solid metal oxide nanoparticles along with complex conjugation procedures. Hence, this challenge inspires the development of metal oxide nanocomposites with improved structure to achieve high loading of lysozyme. In this project, copper zinc oxide (CuZnO) nanospheres with porous and core shell structure was prepared by the hard templating method. The core shell nanostructure was obtained at 320°C where the APF polymer template has been completely decomposed. The nanospheres have the size of 414 nm, total pore volume of 0.08 cm³/g and range of pore size between 12 nm to 30 nm. The CuZnO nanospheres can effectively load lysozyme up to 64% by adsorption method. These findings provided new insight on the use of porous metal oxide nanocomposite as an efficient antimicrobial enzyme carrier.

Keywords: copper zinc oxide; nanocomposites; core shell; porous; nanostructure; lysozyme; carrier.

DOI: 10.1504/IJNP.2021.118107

International Journal of Nanoparticles, 2021 Vol.13 No.3, pp.159 - 173

Received: 28 Jun 2020
Accepted: 15 Feb 2021
Published online: 12 Oct 2021 *

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