Title: Functional tests of an in vitro maintenance system prototype type organ-on-chip for skeletal muscle

Authors: Diana S. Ruvalcaba Martínez; Christian Chapa González; Eduardo I. Acosta Gómez; Esmeralda S. Zuñiga Aguilar

Addresses: Electrical and Computing Engineering Department, Universidad Autónoma de Ciudad Juárez, Cd. Juárez Chih., CP 32310, México ' Electrical and Computing Engineering Department, Universidad Autónoma de Ciudad Juárez, Cd. Juárez Chih., CP 32310, México ' Electrical and Computing Engineering Department, Universidad Autónoma de Ciudad Juárez, Cd. Juárez Chih., CP 32310, México ' Electrical and Computing Engineering Department, Universidad Autónoma de Ciudad Juárez, Cd. Juárez Chih., CP 32310, México

Abstract: Due to the necessity to develop new systems which maintain the proper conditions for cell growth and cell interaction in vitro, this work has focused on the characterisation of two models of maintenance system prototypes type organ-on-chip skeletal muscle tissue of neonatal mouse. Cell growth was monitored for four days. The images of the cells were obtained through an optical microscope to measure the variations of the alignment angle, the analysis was performed using the ImageJ software. It is concluded that the two OoC prototype models delimit and help cell alignment, but because the surface of the microchannels was not homogeneous or completely flat, adding to this the depth factor, the cell proliferation was affected. As a result, we demonstrated that the proposed organ-on-chip system promotes cell alignment.

Keywords: organ-on-chip; OoC; cell alignment; microchannels.

DOI: 10.1504/IJBNN.2020.116323

International Journal of Biomedical Nanoscience and Nanotechnology, 2020 Vol.4 No.3/4, pp.191 - 205

Received: 06 Aug 2020
Accepted: 24 Sep 2020
Published online: 20 Jul 2021 *

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