Title: Application of SERS active substrate prepared by nanotechnology in ophthalmic care

Authors: Lili Lin; Jiarui Guo; Yang Hao

Addresses: Department of Ophthalmology, The First affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China ' Department of Ophthalmology, The First affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China ' Department of Ophthalmology, The First affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, 150001, China

Abstract: The application of nanomaterials in ocular drug delivery is mainly concentrated in nano-controlled release systems. Due to the unique properties of nanomaterials, the use of nanomaterials to carry drugs for treating eye diseases has great advantages compared with traditional drug delivery methods. The nano-formulation of the drug has higher bioavailability and lower side effects. Therefore, the nano-controlled release system has a good application prospect in ophthalmology. In this paper, Fe₂O₃ magnetic nanoparticles were first prepared by co-deposition, and then the nanoparticles were clad with silica to prepare Fe₂O₃ @SiO₂ core-shell nanostructures. Secondly, an ordered array surface-enhanced Raman scattering (SERS) active substrate was formed on the surface of the porous layer of porous anode aluminium oxide (PAA) and the barrier layer using vacuum electron beam evaporation technology using PAA films as templates. Then, the shell thickness of Fe₂O₃@SiO₂ and the number of coated magnetic particles were controlled by controlling the dosage of ethyl orthosilicate, and a layer of SERS active gold nanoshells was grown after further adsorption of gold seeds to prepare a composite SERS active substrate. Enhanced performance magnetic nanomaterial Fe₂O₃@SiO₂@PAA @Au. Finally, the SERS spectrum and fluorescence quenching characteristics of ocular cells adsorbed on the substrate were detected and analysed. The results show that the magnetic nanomaterial Fe₂O₃@SiO₂@PAA @Au with enhanced performance of SERS active substrate has a good enhancement effect on the Raman scattering signal of eye cells, and to a certain extent, it can eliminate the interference of the fluorescent background. Moreover, the ordered array of SERS active substrates not only has a high SERS enhancement ability and ability to quench fluorescence, but also does not have interference peaks of oxalate impurities remaining in the PAA film, which can obtain more detailed information of the Raman scattering spectrum of eye cells to achieve eye care.

Keywords: SERS active substrate; nanotechnology; ophthalmic care; nanomaterials; eye diseases.

DOI: 10.1504/IJNT.2020.110715

International Journal of Nanotechnology, 2020 Vol.17 No.2/3/4/5/6, pp.202 - 219

Published online: 28 Oct 2020 *

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