Title: Investigation on staging of breast cancer using miR-21 as a biomarker in the serum

Authors: Bindu Salim; M.V. Athira; A. Kandaswamy; Madhulika Vijayakumar

Addresses: Nanotech Research Innovation and Incubation Centre, PSG Institute of Advanced Studies, Coimbatore-641004, Tamilnadu, India ' Nanotech Research Innovation and Incubation Centre, PSG Institute of Advanced Studies, Coimbatore-641004, Tamilnadu, India ' Industrial Research and Development, PSG College of Technology, Coimbatore-641004, Tamilnadu, India ' Department of Oncology, PSG Institute of Medical Science and Research, Coimbatore-641004, Tamilnadu, India

Abstract: Circulating microRNAs (miRNA) are a novel class of stable, minimally invasive disease biomarkers that are valuable in diagnostics and therapeutics. miR-21 is an oncogenic miRNA that regulates the expression of multiple cancer-related target genes and it is highly expressed in the patients' serum suffering from breast cancer. The focus of the present study was on measuring the expression profile of the most significantly up-regulated miR-21 in breast cancer patients' serum to evaluate their correlation with the clinical stage of cancer by using a molecular beacon probe. miR-21 expression was also quantitatively analysed by TaqMan real-time PCR techniques. Ten serum samples from the confirmed breast cancer patients and one healthy control sample were used for the evaluation of miR-21 gene expression. The expression levels of miR-21 were significantly higher in breast cancer serum samples compared to healthy control samples with significant differences corresponding to clinical stages of II, III, and IV. The findings indicate that serum miR-21 would serve as a potential marker for therapeutic regimes as well as monitoring the patient status by a simple blood test.

Keywords: breast cancer; biomarker; miR-21; clinical stage; real-time PCR.

DOI: 10.1504/IJBET.2017.10021948

International Journal of Biomedical Engineering and Technology, 2020 Vol.33 No.3, pp.211 - 221

Received: 29 Jun 2017
Accepted: 13 Sep 2017

Published online: 17 Jun 2020 *

Full-text access for editors Access for subscribers Purchase this article Comment on this article