Title: The effect of biochar and nutrients on efficiency of anthracene remediation in soil by Bacillus subtilis and Streptomyces sp

Authors: Vahdat Jahed; Farhad Jahed; Sumayyah Najirad; Manoochehr Farjaminezhad

Addresses: Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio, USA ' Department of Environmental Sciences, Islamic Azad University of Ardabil Branch, Ardabil, Iran ' Department of Environmental Sciences, Islamic Azad University of Ardabil Branch, Ardabil, Iran ' Department of Chemistry, Ardebil Branch, Islamic Azad University, Ardebil, Iran

Abstract: Bioremediation has been found as an effective way to remove PAHs contaminants. Biochar and nutrients as effective factors can raise removal percent. The effect of these two factors was investigated for remediation of anthracene in the presence and absence of two microorganisms named Bacillus subtilis and Streptomyces sp. To study biochar impression, a sandy loam soil was mixed with biochar (10% w: w). Nutrients effect was studied by addition of K2HPO4 and NH4NO3 to the soil. Anthracene degradation was quantified using gas chromatography. The highest rate of removal was observed for the incubation of Bacillus sabtilis with the addition of biochar and nutrients. Bacillus sabtilis had decreased the soil Anthracene by 92%, and Streptomyces sp. bacteria with the same condition could degrade by 90.71% after 90 days. This study shows, the addition of biochar and nutrients could increase the anthracene bioremediation by 11 and 7% respectively.

Keywords: anthracene; bioremediation; Bacillus subtilis; Streptomyces sp.; biochar; nutrients; gas chromatography.

DOI: 10.1504/IJEWM.2020.104349

International Journal of Environment and Waste Management, 2020 Vol.25 No.1, pp.97 - 111

Received: 17 Sep 2018
Accepted: 29 Dec 2018

Published online: 26 Dec 2019 *

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