Authors: Farzin Asghari Sana; Farshad Farhadnia; Ahmad Salmanogli
Addresses: Solid Tumor Research Center, School of Allied Medical Sciences, Urmia University of Medical Science, Iran ' Computer Science Center, University of Tabriz, Tabriz, Iran ' Electrical and Electronics Engineering Department, Faculty of Engineering, Çankaya University, Ankara, Turkey
Abstract: Using functionalised nanoparticles in nanobiomedical applications cause to raise questions about unintentional effect of such powerful agents on the human body. In other words, the study of nanoparticle toxicity can be considered as a curtail key for the biological applications. In this study, the core/shell nanoparticles such as Si/Au and SiO2/Au were synthesised and functionalised with some biological elements. For satisfying some medical standards and biomedical critical test conditions, a few in-vitro assays as cytotoxicity and haemolysis should be done. For this reason, cytotoxicity and haemolytic effects of the functionalised nanoparticles such as Si/Au/biotin and SiO2/Au/biotin and their derivatives were evaluated on Hep-G2 cells and humane red blood samples. It is shown that cytotoxicity and haemolysis effects of the all synthesised nanoparticles are concentration-dependent. Also, the results are shown that most cytotoxicity and haemolytic effects are observed for Si/Au and SiO2/Au without biotin groups after 48 hours and 30 minutes, respectively.
Keywords: silicon core/shell nanoparticle; nanocytotoxicity; MTT cytotoxicity assay; haemolysis.
International Journal of Nanoparticles, 2019 Vol.11 No.4, pp.283 - 293
Received: 28 Feb 2018
Accepted: 15 Mar 2019
Published online: 17 Dec 2019 *