Title: Development of bacterial cellulose biomaterial: preparation and establishment of cytotoxicity for eukaryotic cells

Authors: T.I. Gromovykh; A.G. Demchenko; E.G. Cheremnykh; N.B. Feldman; A.V. Lyundup; M.V. Balyasin; O.I. Kiselyova; P.S. Gromovykh; S.V. Lutsenko

Addresses: Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Advanced Cell Technology, Institute of Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Mental Health Research Center of RAS, Moscow, Kashirskoe Shosse, 34, 115522, Russia ' Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Advanced Cell Technology, Institute of Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Advanced Cell Technology, Institute of Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Physics, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow, 119991, Russia ' Federal State Budgetary Establishment of Science Federal Research Center for Food, Biotechnology and Food Safety, 109240, Russia ' Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia

Abstract: Bacterial cellulose is a biomaterial produced by Gluconacetobacter hansenii bacteria with peculiar properties. The aim of this research was to assess three methods for washing bacterial cellulose from producer cells: 5% SDS, RIPA and RIPA with the addition of nucleases. Atomic force microscopy showed that all methods provide the removal of bacterial cells. The authors carried out a quantitative estimation of residual DNA on the bacterial cellulose matrix and demonstrated a significant difference (p < 0.05) in the DNA content between the group of RIPA with the addition of nucleases and the non-washed matrix. The authors evaluated the cytotoxicity of the extracts and the matrix of bacterial cellulose in regard to protozoa (Tetrahymena) and the mammalian cell culture (NIH/3T3), respectively. The results from the assay with the Biolat device and the MTT assay indicated that the samples are not cytotoxic. The Live/Dead assay of NIH/3T3 showed that bacterial cellulose is an adhesive matrix in regard to mammalian cells and maintains cell viability. In accordance with the authors' data, a bacterial cellulose matrix may provide with washing 5% SDS and RIPA; however, the addition of nucleases is necessary to reduce residual bacterial DNA.

Keywords: bacterial cellulose; biomaterial; regenerative medicine; Gluconacetobacter hansenii; Tetrahymena; NIH/3T3; cytotoxicity; cell viability.

DOI: 10.1504/IJNT.2019.102395

International Journal of Nanotechnology, 2019 Vol.16 No.1/2/3, pp.87 - 99

Published online: 12 Sep 2019 *

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