Title: Comparative study of the pathogenic variability of some Morrocan isolates of Botrytis cinerea

Authors: Ahlem Hamdache; Ahmed Lamarti

Addresses: Department of Biology, Faculty of Sciences of Tetuan, Abdelmalek Essaâdi University, Mhannech II. 93002, Tetuan, Morocco ' Department of Biology, Faculty of Sciences of Tetuan, Abdelmalek Essaâdi University, Mhannech II. 93002, Tetuan, Morocco

Abstract: Botrytis cinerea attacks economically important crops such as strawberries producing grey mould. The aim of this work was to select the most pathogenic isolate of B. cinerea to use it in biological control tests. For this purpose, we have studied, in vitro, the growth and the sporulation of some isolates of B. cinerea in different nutrient media. Then we have investigated the effect of some bacterial isolates on these botrytis populations isolated from strawberry fruits. Seven strains were isolated from samples of strawberry fruit harvested from fields; four of them, are originated from strawberry fields of Loukkos and three are originated from fields of Moulay Bouselham. Others are isolated from postharvest strawberry. Among all of the isolates tested, B. cinerea Bt7 originated from fields of Zlaoula (Loukkos) was the most important isolate with maximum growth and sporulation. Bt7 was the most resistant isolate to the antifungal activity of the antagonistic bacteria.

Keywords: nutrient media; mycelial growth; sporulation; pathogenicity; Moulay Bouselham; Loukkos; antifungal activity; antagonism.

DOI: 10.1504/IJSAMI.2019.101675

International Journal of Sustainable Agricultural Management and Informatics, 2019 Vol.5 No.2/3, pp.148 - 157

Received: 12 Nov 2018
Accepted: 07 Apr 2019
Published online: 20 Aug 2019 *

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