Title: Effect of nanoparticles on antioxidant activity of alfalfa seedlings

Authors: Yurong Wang; Jipeng Mo

Addresses: School of Biomedicine and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi, 117004, Liaoning, China ' School of Biomedicine and Chemical Engineering, Liaoning Institute of Science and Technology, Benxi, 117004, Liaoning, China

Abstract: Background: Alfalfa, a perennial legume from the family Fabaceae, is well known for its uses in agriculture and medicine. It is utilised as a cover crop, green manure, and for hay, silage, and grazing. It has been long utilised as a successful treatment for digestive problems, cardiac issues, and neurological issues. Small antioxidant compounds and enzymes help alfalfa become a stress-tolerant plant. Objective: Research investigates the impact of different NP, specifically TiO₂ and Fe₂O₃, on the antioxidant activity of alfalfa seedlings. Methods:To assess the effects of these NPs on the formation of ROS, antioxidant enzyme activities, and general plant health, seedlings were subjected to various doses of these NPs. Results: The result shows that by reducing the formation of ROS and increasing reduced GSH, alfalfa improves cell viability of p-value < 0.001 by reducing DNA fragmentation and blocking the apoptotic pathway. Conclusion: Research emphasises alfalfa's potential as an adjuvant in agriculture involving NP procedures and as a preventive agent against oxidative stress generated by NP.

Keywords: antioxidant activity; TiO₂; titanium oxide; Fe₂O₃; iron oxide; alfalfa; ROS; reactive oxygen species.

DOI: 10.1504/IJNBM.2026.153198

International Journal of Nano and Biomaterials, 2026 Vol.11 No.3, pp.199 - 219

Received: 10 Sep 2024
Accepted: 23 Mar 2025
Published online: 29 Apr 2026 *

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