Title: Preparation and characterisation of antibacterial silver-containing nanofibres for wound healing in diabetic mice

Authors: Thanaa Ibrahim Shalaby; Nivan Mahmoud Fekry; Amal Sobhy El Sodfy; Amel Gaber El Sheredy; Maisa El Sayed Sayed Ahmed Moustafa

Addresses: Medical Biophysics Department, Medical Research Institute, Alexandria University, Egypt ' Medical Biophysics Department, Medical Research Institute, Alexandria University, Egypt ' Pathology Department, Medical Research Institute, Alexandria University, Egypt ' Microbiology Department, Medical Research Institute, Alexandria University, Egypt ' Medical Biophysics Department, Medical Research Institute, Alexandria University, Egypt

Abstract: Impaired wound healing is a common complication of diabetes mellitus. Nanofibre can be served as powerful tools in advanced wound care management. The aim of this work is to evaluate the role of antibacterial nanofibres by incorporating silver nanoparticles into celluose acetate nanofibres as wound dressing on excisional wound healing in diabetic mice. Celluose acetate nanofibres were prepared by electrospinning technique. The prepared nanofibres were characterised using scanning electron microscope (SEM), and Fourier-transform infrared (FTIR) spectrophotometer. The antimicrobial activity was tested against Gram-positive and Gram-negative bacteria. A full thickness of the excision wound of circular area 95 mm² and 2 mm depth was created. Wound dressed by silver containing nanofibres, the wound closure rate were assessed, skin wounds were processed for histopathological examination and comparing the mechanical properties of healed skin. It was found that, wounds dressed with silver containing nanofibres showed marked increase in collagen production which improve the skin mechanical properties.

Keywords: antibacterial nanofibres; electrospinning; wound dressing; antibacterial properties; wound healing; diabetes mellitus; nanotechnology; silver nanoparticles; celluose acetate nanofibres; diabetic mice; wound care management; wound closure rate; skin wounds; collagen production.

DOI: 10.1504/IJNP.2015.070346

International Journal of Nanoparticles, 2015 Vol.8 No.1, pp.82 - 98

Received: 30 Apr 2014
Accepted: 04 Nov 2014
Published online: 02 Jul 2015 *

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