Title: Comparison investigation of several hydrogen peroxide biosensor based on the direct electrochemistry of horseradish peroxidase immobilised on self-assembled monolayers
Author: Chuanyin Liu
Address: School of Chemical Engineering and Food Science, Hubei University of Arts and Science, Xiangyang 441053, China; Key Laboratory Low-Dimensional of Optoelectronic Materials and Device, Hubei University of Arts and Science, Hubei Province, Xiangyang 441053, China
Abstract: Horseradish peroxidase was immobilised onto self-assembled monolayers (SAMs) by electrostatic adsorption, acylimide and Sciff base covalent-linkage, respectively. The effects of different immobilised methods on the direct electrochemistry of HRP and electrocatalysis towards H2O2 have been evaluated. The experimental results reveal that the direct electrochemistry of HRP can be achieved by the method of acylimide linkage. The electron transfer coefficient α and rate constant ks have been calculated to be 0.70 and 33.88 s-1 for HRP-GSH/Au, 0.52 and 11.35 s-1 for HRP-Cys/Au, respectively. The response is linear to H2O2 concentration from 1 µM to 1.4 mM and lower detection limit of 0.46 µM for HRP-GSH/Au, 1.5µM to 1.2 mM and lower detection limit of 0.76 µM for HRP-Cys/Au. The biosensor showed longer stability and favourable reproducibility and anti-interfering ability.
Keywords: hydrogen peroxide biosensors; HRP; direct electrochemistry; self-assembled monolayers; SAMs; horseradish peroxidase; electron transfer coefficient; rate constant.
Int. J. of Nanomanufacturing, 2014 Vol.10, No.1/2, pp.42 - 55
Submission date: 28 Nov 2012
Date of acceptance: 14 Mar 2013
Available online: 01 Feb 2014