Title: Comparison investigation of several hydrogen peroxide biosensor based on the direct electrochemistry of horseradish peroxidase immobilised on self-assembled monolayers

Authors: Chuanyin Liu

Addresses: School of Chemical Engineering and Food Science, Hubei University of Arts and Science, Xiangyang 441053, China; Key Laboratory Low-Dimensional of Optoelectronic Materials and Device, Hubei University of Arts and Science, Hubei Province, Xiangyang 441053, China

Abstract: Horseradish peroxidase was immobilised onto self-assembled monolayers (SAMs) by electrostatic adsorption, acylimide and Sciff base covalent-linkage, respectively. The effects of different immobilised methods on the direct electrochemistry of HRP and electrocatalysis towards H₂O₂ have been evaluated. The experimental results reveal that the direct electrochemistry of HRP can be achieved by the method of acylimide linkage. The electron transfer coefficient α and rate constant ks have been calculated to be 0.70 and 33.88 s-1 for HRP-GSH/Au, 0.52 and 11.35 s-1 for HRP-Cys/Au, respectively. The response is linear to H₂O₂ concentration from 1 µM to 1.4 mM and lower detection limit of 0.46 µM for HRP-GSH/Au, 1.5µM to 1.2 mM and lower detection limit of 0.76 µM for HRP-Cys/Au. The biosensor showed longer stability and favourable reproducibility and anti-interfering ability.

Keywords: hydrogen peroxide biosensors; HRP; direct electrochemistry; self-assembled monolayers; SAMs; horseradish peroxidase; electron transfer coefficient; rate constant.

DOI: 10.1504/IJNM.2014.058996

International Journal of Nanomanufacturing, 2014 Vol.10 No.1/2, pp.42 - 55

Received: 29 Nov 2012
Accepted: 14 Mar 2013
Published online: 17 May 2014 *

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