Enhanced sensitivity to Botulinum type A neurotoxin of human neuroblastoma SH-SY5Y cells after differentiation into mature neuronal cells Online publication date: Sat, 06-Aug-2011
by C. Rasetti-Escargueil, C.B. Machado, R. Preneta-Blanc, R.A. Fleck, D. Sesardic
The Botulinum J. (TBJ), Vol. 2, No. 1, 2011
Abstract: Research on more sensitive cell based assays is essential to assess neutralising activities of Botulinum neurotoxins antibodies. A reproducible and robust differentiation protocol was applied on human neuroblastoma SH-SY5Y cells generating neuronal cultures stable for 2–3 weeks. The presence of specific neuronal markers was assessed with labelled antibodies. Accurate and reproducible noradrenaline release levels were obtained which were significantly higher in differentiated cells and correlated with vesicle turnover assessments. The suitability of differentiated SH-SY5Y cells for detecting low levels of Botulinum type A and for toxin neutralisation assay was evidenced using functional end points relevant to Botulinum neurotoxicity.
Online publication date: Sat, 06-Aug-2011
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