Ex vivo analysis of the oral epithelium by high-wavenumber Raman spectroscopy
by Roberto Valdés; Stefan Stefanov; Stefano Chiussi; Pío González; Miriam López-Álvarez
International Journal of Biomedical Engineering and Technology (IJBET), Vol. 24, No. 2, 2017

Abstract: Raman spectroscopy at wavenumber region of 400-1800 cm−1 is an already proved promising technology for objective clinical evaluation of biological tissues to obtain their molecular fingerprint. The study of the high-wavenumber region (2800-3100 cm−1) is also of interest to significantly shorten the diagnosis time due to the intense Raman signal in a narrower range. This work studies parameters dependence, Raman saturation in depth and repeatability of tissue measurements at this region and proposes three indices for quantitative diagnose in oral epithelium. Sixteen oral mucosa biopsies in different thicknesses were evaluated in varying laser powers. The non-saturation of Raman signal up to 120 μm in depth was demonstrated. Three peaks (2874, 2926, 3056 cm−1) attributed to functional groups of interest in oral mucosa were identified from the Raman band deconvolution. The high-wavenumber correspondence with the fingerprint region (400-1800 cm−1) was proven and three diagnose indices proposed to quantitatively evaluate oral epithelium tissue in routinely clinical practice.

Online publication date: Tue, 20-Jun-2017

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Biomedical Engineering and Technology (IJBET):
Login with your Inderscience username and password:

    Username:        Password:         

Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com