Effect of three non-protein thiols on CHO cells exposed to low doses of X-radiation
by J.C. De Luca; D.M. López-Larraza
International Journal of Low Radiation (IJLR), Vol. 9, No. 2, 2013

Abstract: The goals of this study were to determine whether cysteamine, β-mercaptohetanol and glutathione protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of each thiol, (c) cells treated with 100 mGy of X-rays and (4) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all thiols were added 30 min before irradiation. Thiols remained until cell sacrifice (18 h). Maximum protection was afforded by cysteamine, the minimum protection was produced by glutathione while β-mercaptohetanol did not show radioprotection effect. These results are consistent with the electrical charges and chemical structure of the three thiols and might be explained by the lower or higher access to DNA.

Online publication date: Sat, 13-Sep-2014

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Low Radiation (IJLR):
Login with your Inderscience username and password:

    Username:        Password:         

Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com