Optimisation of process variables for microbial degradation of phenol by binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens using response surface methodology Online publication date: Sat, 09-Oct-2010
by Samuel E. Agarry, Thomas O.K. Audu, Bamidele O. Solomon
International Journal of Environment and Pollution (IJEP), Vol. 43, No. 1/2/3, 2010
Abstract: The removal efficiency of phenol from aqueous solutions was measured using an indigenous freely suspended binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens. Experiments were performed as a function of temperature (25–45°C), aeration (1.0–3.5 vvm) and agitation (200–600 rpm). Optimisation of these three process parameters for phenol biodegradation was studied. Statistically designed experiments using response surface methodology were used to get more information about the significant effects and the interactions between the three parameters. A 2³ full-factorial central composite design followed by multistage Monte Carlo optimisation technique was employed for experimental design and analysis of the results. The optimum process conditions of temperature 30°C, aeration 3.0 vvm and agitation 300 rpm were recognised for maximising phenol degradation (removal). Maximum removal efficiency of phenol was achieved (100%) at the optimum process conditions.
Online publication date: Sat, 09-Oct-2010
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Environment and Pollution (IJEP):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email email@example.com