Authors: Sekhar Talluri
Addresses: Department of Biotechnology, GIT, GITAM University, Visakhapatnam, Andhra Pradesh, India
Abstract: Bacteriocins are highly potent anti-microbial polypeptides and proteins produced by bacteria. They are rapidly degraded in the environment after their use, due to their proteinaceous nature. Some bacteriocins are used as preservatives in foods. Native and engineered bacteriocins are of potential interest as replacements for conventional antibiotics that are losing their efficacy due to the development of antibiotic-resistant strains. Aureocin A53 is a class II bacteriocin. It is a broad spectrum microbicide, with demonstrated ability to inhibit the growth of methicillin-resistant Staphylococcus aureus. Validated computational protein design tools have been used for reengineering of the aureocin A53 sequence to produce novel sequence variants of the bacteriocins aureocin A53 and lacticin Q. The novel proteins are expected to possess an altered spectrum of bactericidal specificity and potency.
Keywords: antibiotic; anti-microbial peptide; bacteriocin; computational protein design; methicillin-resistant Staphylococcus aureus; microbicide; molecular modelling; MRSA; protein engineering.
International Journal of Bioinformatics Research and Applications, 2019 Vol.15 No.2, pp.129 - 143
Available online: 06 May 2019 *Full-text access for editors Access for subscribers Purchase this article Comment on this article