Title: Production of biodiesel feedstock - microbial lipid from slaughterhouse wastewater

Authors: Thirulogachandar Asokan; Jayanthi Singaram; Priyadharshni Vairava Sundaram

Addresses: Department of Environmental Engineering, Government College of Technology, Coimbatore, India ' Department of Civil Engineering, Government College of Engineering, Theni, India ' Department of Environmental Engineering, Government College of Technology, Coimbatore, India

Abstract: Biodiesel is an alternative diesel fuel, which can be synthesised from renewable biological sources. Lipid production using carbon source in wastewater is an emerging process as it purely depends on waste source. In the present study, the ability of Yarrowia lipolytica to accumulate lipids using slaughterhouse wastewater as substrate was investigated. Using raw wastewater as substrate, maximum lipid content (0.43 g/L) and biomass (1.2 g/g) were obtained. Various pre-treatment methods like acid, alkaline, heat, activated carbon and sawdust treatment were performed and two-fold increase in C/N ratio was observed in combined pre-treatment of sawdust with KOH. Using pretreated wastewater, lipid accumulation was enhanced to 32% with lipid content of 0.64 g/L. Results of this study conclude that the pre-treated slaughterhouse wastewater can be employed as a better feedstock for lipid production using Yarrowia lipolytica.

Keywords: oleaginous microorganisms; Yarrowia lipolytica; slaughterhouse wastewater; C/N ratio; pre-treatment.

DOI: 10.1504/IJESD.2018.094035

International Journal of Environment and Sustainable Development, 2018 Vol.17 No.2/3, pp.113 - 123

Received: 22 Mar 2017
Accepted: 31 Jan 2018
Published online: 13 Aug 2018 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article