Title: EDTA and citrate impact on heavy metals phytoremediation using paulownia hybrids

Authors: K. Miladinova-Georgieva; Katya Ivanova; Teodora Georgieva; Maria Geneva; Petar Petrov; Ira Stancheva; Yuliana Markovska

Addresses: Department of Plant-Soil Interaction, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria ' Department of Plant Physiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria ' Biotree Ltd., 7 Shosse Bankya Str., Sofia, Bulgaria ' Department of Plant-Soil Interaction, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria ' Department of Plant-Soil Interaction, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria ' Department of Plant-Soil Interaction, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria ' Department of Plant Physiology, Faculty of Biology, University of Sofia, Sofia, Bulgaria

Abstract: The influence of the EDTA and citrate addition to the industrially polluted soil on phytoextraction of heavy metals, leaf anatomy, and gas exchange parameters of two Paulownia hybrid lines (Paulownia tomentosa x fortunei - TF 01 and Paulownia elongata x fortunei - EF 02) was evaluated. Both lines were accumulators of Cu, Zn and Cd because bioaccumulation factor values were higher than 1.00. The levels of heavy metal accumulation in shoots allowed supposing that both hybrids may be used for phytoremediation since transfer factors for Pb and Zn in hybrid - TF 01 were higher than 1.00, while in hybrid - EF 02 - for Zn only. Application of EDTA produced heavy metal accumulation in hybrid lines higher than those obtained with citrate. Treatment with 1 mM EDTA displayed a protective effect on leaf development in both lines and overcame damages in its morphological structure caused by heavy metal stress.

Keywords: Paulownia elongata x fortunei - EF 02; Paulownia tomentosa x fortunei - TF 01; phytoextraction; mesophyll; thickness; gas exchange.

DOI: 10.1504/IJEP.2018.092985

International Journal of Environment and Pollution, 2018 Vol.63 No.1/2, pp.31 - 46

Received: 21 Oct 2016
Accepted: 25 Mar 2017

Published online: 26 Jun 2018 *

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