Title: Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis

Authors: Tur Rahardjo; Wiwin Mailana; Teja Kisnanto; Darlina; Siti Nurhayati; Devita Tetriana; Dwi Ramadhani; Mukh Syaifudin

Addresses: Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia ' Nuclear Medicine Technique and Radiation Biology Division, Center for Technology of Radiation Safety and Metrology (PTKMR), National Nuclear Energy Agency (BATAN), Jl. Lebak Bulus Raya no. 49 Jakarta 12440, Indonesia

Abstract: Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.

Keywords: comet assay; DNA damage; high background radiation area; lymphocytes; Mamuju.

DOI: 10.1504/IJLR.2017.087691

International Journal of Low Radiation, 2017 Vol.10 No.4, pp.314 - 323

Received: 12 Jan 2017
Accepted: 16 Jul 2017
Published online: 30 Oct 2017 *

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