Authors: Maryam Moazami-goodarzi; Reza Fotouhi-Ardakani; Ali Afgar
Addresses: Molecular and Medicine Research Center, Biotechnology Department, Arak University of Medical Sciences, Arak, Iran ' Cellular and Molecular Research Center, Clinical Laboratory Science Department, Qom University of Medical Sciences, Qom, Iran ' Research Center for Hydatid Disease in Iran, Parasitology Department, Kerman University of Medical Sciences, Kerman, Iran
Abstract: To strength the potency of vaccines against infectious diseases, vaccines should be able to activate multiple arms of the immune system including innate and adaptive immunity. Moreover, genetic variation often occurs in infectious microorganisms can reduce the effectiveness of vaccines. Current vaccines and methods to immunisation against human papilloma virus, as a leading cause of different cancers, stimulate immune system moderately. In this study, to obtain highly effective responses, conserved Th and B-cell epitopes of L1 and CTL and Th epitopes of E7 are fused to helicobacter pylori NapA which is responsible for activation of innate immunity and direction favourable response to appropriate state. Finding the best conserved epitopes in order to induce better immune system may increase promising approach to achieve high-level immunity against infectious agents such as human papilloma virus (HPV). Thus, elevated immunity can be able to eradicate HPV vigorously. Designed construct can be useful as a peptide-based subunit vaccine to confer both preventive and therapeutic effects against all HPV16 subtypes.
Keywords: conserved epitopes; construct designing; human papilloma virus; NapA; peptide-based vaccine; TLR-dependent adjuvants.
International Journal of Bioinformatics Research and Applications, 2017 Vol.13 No.3, pp.223 - 241
Received: 12 May 2016
Accepted: 11 Dec 2016
Published online: 08 Aug 2017 *