Title: Understanding the role of Salmonella pathogenic island 1 (SPI-I) and host-pathogen interaction for typhoid using system biology approach
Authors: Devender Arora; Kumari Jyoti; Ajeet Singh
Addresses: Department of Biotechnology, G.B. Pant Engineering College, Pauri, Uttarakhand, India ' Department of Biotechnology, G.B. Pant Engineering College, Pauri, Uttarakhand, India ' Department of Biotechnology, G.B. Pant Engineering College, Pauri, Uttarakhand, India
Abstract: Salmonella, a Gram-negative bacterium species capable of causing a variety of food-and water-borne infections. Salmonella is reported as multiple drug resistant to various drugs which makes pharmaceutical industries work and develop new strategies for producing effective antibiotics against them. With the molecular advancement and better understanding of their pathway architecture, a channel for developing better antibiotics is open. Here, we tried to understand the enzyme kinetics of pathogenicity island in Salmonella and host-pathogen relationship by system biology approach. With taking consideration, we have identified in the pathogenic island, control over HilA is governed by HilC, HilD, RtsA combinatorial effect and concentration of each factor directly related during the time interval. It was observed that higher concentration of HilD, SipA, SipD in the diseased state leads to enhance the level of CDC42 and Arp2/3 complex protein expression during pathogen infect the cell. This study opens target for developing future drugs against Salmonella typhi infection.
Keywords: combinatorial effect; host-pathogen; multidrug resistance; pathogenicity island; system biology.
International Journal of Bioinformatics Research and Applications, 2017 Vol.13 No.3, pp.187 - 199
Received: 04 Jun 2016
Accepted: 11 Dec 2016
Published online: 08 Aug 2017 *