Title: Definition of optimum basic nutrients ratio in media for bioethanol production with immobilised yeast cells

Authors: Zorana Rončević; Jelena Dodić; Jovana Grahovac; Siniša Dodić; Bojana Bajić; Damjan Vučurović; Ivana Tadijan

Addresses: Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia ' Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 21000, Serbia

Abstract: The production of bioethanol as an alternative to fossil fuel has been increasing owing to growing biofuel demand. Combination of yeast immobilisation and media optimisation has become a popular technique for the improvement of bioethanol technology. The aim of this study was the optimisation, by response surface methodology, of sodium alginate concentration and nitrogen and phosphorus content in media for bioethanol production using immobilised Saccharomyces cerevisiae. Ratio of carbon, nitrogen and phosphorus (C : N : P) in media was calculated based on optimum factors values and initial glucose content. The developed model predicts that maximum product concentration, minimum 'eluted' yeast cells number and minimum nutrients content are achieved when the sodium alginate concentration is 71.99 g/L and C : N : P ratio is 50 : 3.5 : 1.3. The obtained results can be used for techno-economic analysis of the process to select optimum fermentation conditions for industrial application.

Keywords: bioethanol production; Saccharomyces cerevisiae; microbial immobilisation; media optimisation; response surface methodology; RSM; basic nutrient ratio; nutrients; immobilised yeast cells; biofuels; sodium alginate; carbon; nitrogen; phosphorus; glucose content; techno-economic analysis; optimum fermentation conditions.

DOI: 10.1504/IJISD.2017.080617

International Journal of Innovation and Sustainable Development, 2017 Vol.11 No.1, pp.53 - 68

Received: 07 Apr 2015
Accepted: 04 Sep 2015

Published online: 01 Dec 2016 *

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