Article: Lack of epithelial-to-mesenchymal transition induction in two bronchial epithelial cell lines after alpha and gamma irradiation Journal: International Journal of Low Radiation (IJLR) 2015 Vol.10 No.2 pp.116 - 133 Abstract: The induction of epithelial-to-mesenchymal transition (EMT) in human lung epithelial cell lines was investigated after α-particle and γ-radiation exposures. We applied TGF-β treatment of cells as positive EMT-controls and tested in parallel if radiation has a potentiating effect on the EMT induction. BEAS-2B and HBEC-3KT cells were irradiated with 5.4 MeV α-particles or γ-rays (<SUP align="right"><SMALL>60</SMALL></SUP>Co, 1.13-1.15 Gy/min) with or without of TGF-β. The cells were harvested three days post treatment and the EMT markers vimentin, fibronectin and E-cadherin were analysed by immunofluorescence staining and Western blotting. The TGF-β treatment-induced EMT in both cell lines in the applied concentrations. We could not prove any clear EMT induction with low or moderate doses of α-particles and γ-rays. No significant additive effect with radiation and TGF-β was observed. We suggest that there might be a different mechanism induced by radiation in bronchial cells after radon and medical exposures that does not involve direct EMT changes. Inderscience Publishers - linking academia, business and industry through research

Title: Lack of epithelial-to-mesenchymal transition induction in two bronchial epithelial cell lines after alpha and gamma irradiation

Authors: Anna Acheva; Elina Eklund; Elina Lemola; Teemu Siiskonen; Virpi Launonen; Meerit Kämäräinen

Addresses: STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland ' STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland ' STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland ' STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland ' STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland ' STUK-Radiation and Nuclear Safety Authority, Environmental Radiation Surveillance, Laippatie 4, 00880 Helsinki, Finland

Abstract: The induction of epithelial-to-mesenchymal transition (EMT) in human lung epithelial cell lines was investigated after α-particle and γ-radiation exposures. We applied TGF-β treatment of cells as positive EMT-controls and tested in parallel if radiation has a potentiating effect on the EMT induction. BEAS-2B and HBEC-3KT cells were irradiated with 5.4 MeV α-particles or γ-rays (⁶⁰Co, 1.13-1.15 Gy/min) with or without of TGF-β. The cells were harvested three days post treatment and the EMT markers vimentin, fibronectin and E-cadherin were analysed by immunofluorescence staining and Western blotting. The TGF-β treatment-induced EMT in both cell lines in the applied concentrations. We could not prove any clear EMT induction with low or moderate doses of α-particles and γ-rays. No significant additive effect with radiation and TGF-β was observed. We suggest that there might be a different mechanism induced by radiation in bronchial cells after radon and medical exposures that does not involve direct EMT changes.

Keywords: EMT; TGF-beta; ionising radiation; bronchial epithelium; epithelial-to-mesenchymal transition; bronchial epithelial cell lines; alpha irradiation; gamma irradiation; human lung; radiation exposure; low radiation; vimentin; fibronectin; E-cadherin; bronchial cells; radon; medical exposure.

DOI: 10.1504/IJLR.2015.074411

International Journal of Low Radiation, 2015 Vol.10 No.2, pp.116 - 133

Received: 02 Mar 2015
Accepted: 12 Jul 2015
Published online: 28 Jan 2016 *

Title: Lack of epithelial-to-mesenchymal transition induction in two bronchial epithelial cell lines after alpha and gamma irradiation

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