Title: Effect of buthionine sulphoximine, cysteine and dithitreitol in cells exposed to low doses of radiation

Authors: J.C. De Luca; D.M. López-Larraza

Addresses: Instituto de Genética Veterinaria (IGEVET), Facultad de Ciencias Veterinarias (UNLP-CONICET), Universidad Nacional de La Plata, CC 296, B1900 La Plata AVW, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917 (C1033AAJ), Ciudad Autónoma de Buenos Aires, Argentina ' Deceased; formerly of: Instituto Multidisciplinario de Biología Celular (IMBICE), Argentina

Abstract: The goal of this study is to determine whether cysteimine, buthionine sulphoximide and dithitreitol protect CHO K1 cells against chromosomal aberrations induced by low doses of X-radiation, as well as whether radiation protection is related to physical properties of those thiols. Experimental design included four different treatments: (a) control, (b) cells treated with 5 mM of buthionine sulphoximide as well as cysteine and dithitreitol, (c) cells treated with 100 mGy of X-rays and (d) cells treated with 5 mM of each thiol and 100 mGy X-rays. In combined treatment, all chemical compounds were added 30 min before irradiation and remained until cell sacrifice (18 h). None of the three compounds studied showed a radioprotector effect. These results are consistent with the electrical charges and chemical structure of the three thiols.

Keywords: cysteine; buthionine sulphoximide; dithitreitol; thiol compounds; CHO K1 cells; chromosome aberrations; low dose radiation; low radiation; radiation protection.

DOI: 10.1504/IJLR.2014.068257

International Journal of Low Radiation, 2014 Vol.9 No.5/6, pp.327 - 334

Received: 23 Apr 2014
Accepted: 22 Aug 2014
Published online: 27 Mar 2015 *

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