Title: An in vitro evidence for caffeic acid, rosmarinic acid and trans cinnamic acid as a skin protectant against γ-radiation

Authors: Faruck Lukmanul Hakkim; Miwa Miura; Naoki Matsuda; Ahmed Suleiman Alharassi; Gilles Guillemin; Motohiro Yamauchi; Girija Arivazhagan; Hyuk Song

Addresses: Chair of Oman Medicinal Plants and Marine Natural Products, DARIS Centre for Scientific Research and Technology Development, University of Nizwa, Oman; Division of Radiation Biology and Protection, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan ' Division of Radiation Biology and Protection, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan ' Division of Radiation Biology and Protection, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan ' Chair of Oman Medicinal Plants and Marine Natural Products, DARIS Centre for Scientific Research and Technology Development, University of Nizwa, Oman ' 3 MND and Neurodegenerative Diseases Research Group, Australian School of Advanced Medicine (ASAM), Macquarie University, New South Wales, Australia ' Division of Radiation Biology and Protection, Center for Frontier Life Sciences, Nagasaki University, Nagasaki, Japan ' 4 Plant Biotechnology Laboratory, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, Tamilnadu, India ' Department of Animal and Food Bioscience, Konkuk University, Chungju 380-701, South Korea

Abstract: Ionising radiation (γ or X-rays) is the mandatory tool to treat cancer despite its detrimental effects in particular on skin cells which lead to severe dermatological diseases and carcinogenesis. Natural antioxidants caffeic acid (CA), rosmarinic acid (RA), trans cinnamic acid (TCA), p-coumaric acid (PCA), and hydroxyphenyllactic acid (HPA) acid are known to be potent anticancer and antioxidant agents. Current study is designed to provide experimental evidence as these compounds offer radiation protection for skin cells. Non-toxic concentrations of CA, RA, TCA, PCA, and HPA were tested for radiation protection, γ-radiation induced intracellular reactive oxygen species (ROS) by flow cytometry and DNA double strand break in human keratinocytes (HaCaT cells) by immunocytochemistry. CA, RA and TCA pre-treatment can protect the HaCaT cells by 40%, 20%, 15% respectively through scavenging γ-radiation induced ROS and decreasing number of post irradiation 53bp1 foci. Inclusion of these compounds in chemo-radiotherapy could facilitate to achieve multiple target protection (i.e. anti-cancer and skin radio protectant).

Keywords: ionising radiation; HaCaT cells; human keratinocytes; caffeic acid; rosmarinic acid; trans cinnamic acid; ROS; reactive oxygen species; DNA repair; skin protection; gamma radiation; cancer treatment; dermatological diseases; carcinogenesis; natural antioxidants; radiation protection; skin cells; p-coumaric acid; hydroxyphenyllactic acid; HPA; immunocytochemistry.

DOI: 10.1504/IJLR.2014.063414

International Journal of Low Radiation, 2014 Vol.9 No.4, pp.305 - 316

Available online: 14 Jul 2014 *

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