Title: In silico analysis of mt 16S rRNA sequences of ocypodid crabs

Authors: Nirmal K. Prasad; Vaibhav Vindal

Addresses: Department of Bioinformatics, Institute of Insilico Biology, Tirupati 517501, India ' Department of Biotechnology and Bioinformatics Infrastructure Facility, University of Hyderabad, Gachibowli, Hyderabad 500046, India

Abstract: The family Ocypodidae comprises of four sub-families Dotillinae, Heloeciinae, Macrophthalminae and Ocypodinae. Ocypodidae mt 16S rRNA sequences were analysed for studying the conserved regions and phylogeny. Conserved regions will be helpful in molecular identification at the sub-family level and in interpretation of the species relationships in a better way. In the current phylogeny, the Ocypodidae family exhibits different lineages, indicating the family is polyphyletic along with its sub-families Dotillinae and Ocypodinae. The genera Ilyoplax of Dotillinae, with three different lineages, and the Uca genera of Ocypodinae, with four different lineages, exhibit polyphyly. Ocypode and Uca show a close relationship within the sub-family Ocypodinae.

Keywords: mt 16S rRNA; ocypodid crabs; sequence analysis; bioinformatics; rRNA sequences; conserved regions; phylogeny; sub-families.

DOI: 10.1504/IJBRA.2013.054692

International Journal of Bioinformatics Research and Applications, 2013 Vol.9 No.4, pp.311 - 322

Received: 31 May 2011
Accepted: 20 Jun 2011

Published online: 18 Sep 2014 *

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