Title: Ultra-sensitive protein detection using single molecule arrays (SiMoA): the potential for detecting single molecules of botulinum toxin
Authors: David C. Duffy
Addresses: Quanterix Corporation, 113 Hartwell Avenue, Lexington, MA 02421, USA
Abstract: Single molecule arrays (SiMoA) make it possible to detect subfemtomolar concentrations of proteins in complex samples. We describe the basis of SiMoA and its potential application in the detection of proteins, such as botulinum neurotoxin (BoNT), from pathogenic bacteria. We also provide a concept for an integrated handheld SiMoA device for the detection of very low concentrations of BoNT in the field.
Keywords: single molecule arrays; SiMoA; single molecules; proteins; digital ELISA; botulinum neurotoxins; BoNTs; microfluidics; botulism; protein detection; clostridium botulinum.
The Botulinum Journal, 2012 Vol.2 No.2, pp.164 - 167
Received: 08 May 2021
Accepted: 12 May 2021
Published online: 30 Oct 2012 *