Title: Effect of three fungicides on in vitro growth of Fusarium solani and Fusarium semitectum, parasitic on Odontesthes bonariensis eggs

Authors: Suani Giovanna Pacheco-Marino; Marta Noemí Cabello; Alfredo Salibián

Addresses: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Buenos Aires, Argentina. ' Instituto de Botánica Carlos Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 53, No. 477, B1900AVJ, La Plata, Argentina; CIC Provincia de Buenos Aires, Calle 526, entre 10 y 11, 1900, La Plata, Argentina. ' CIC Provincia de Buenos Aires, Calle 526, entre 10 y 11, 1900, La Plata, Argentina; Departamento de Ciencias Básicas (PRODEA-INEDES), Universidad Nacional de Luján, Casilla de Correo 221, B6700ZBA, Luján, Argentina

Abstract: An in vitro evaluation of the effect of three fungicides on the growth rate (GR) of Fusarium solani and Fusarium semitectum was performed. The tested fungicides, commonly used in fish farming, were sodium chloride (NaCl), methanol-stabilised formaldehyde (F) and malachite green (MG). Both Fusarium species were isolated from eggs of the Argentinean pejerrey Odontesthes bonariensis (Valenciennes, 1835). The concentrations of NaCl and MG at which growth of both species was inhibited were higher than the respective mean 96h median lethal concentrations (LC50-96h) for silverside eggs. In the case of F, the growth inhibition (GI) of F. solani was achieved also at concentrations higher than the respective LC50-96h. Only for F. semitectum a complete GI was observed at a concentration lower than the LC50-96h. This indicates that the efficacy of these substances is not adequate for treatment of fungal infections of silverside eggs.

Keywords: Odontesthes bonariensis; silverside eggs; Fusarium spp.; fungicides; in-vitro growth inhibition; sodium chloride; methanol-stabilised formaldehyde; malachite green; median lethal concentrations; LC50-96h; growth rate; fish farming; fungal infections; environmental pollution.

DOI: 10.1504/IJENVH.2011.044114

International Journal of Environment and Health, 2011 Vol.5 No.4, pp.293 - 303

Available online: 09 Dec 2011 *

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