Title: LIPABASE: a database for |true| lipase family enzymes

Authors: Abdelmonaem Messaoudi; Hatem Belguith; Imen Ghram; Jeannette Ben Hamida

Addresses: Unite de Proteomie Fonctionnelle and Biopreservation Alimentaire, Institut Superieur des Sciences Biologiques Appliquees de Tunis, Universite Tunis El Manar, Rue Zouheir ESSAFI 1007, Tunisie. ' Unite de Proteomie Fonctionnelle and Biopreservation Alimentaire, Institut Superieur des Sciences Biologiques Appliquees de Tunis, Universite Tunis El Manar, Rue Zouheir ESSAFI 1007, Tunisie. ' Unite de Proteomie Fonctionnelle and Biopreservation Alimentaire, Institut Superieur des Sciences Biologiques Appliquees de Tunis, Universite Tunis El Manar, Rue Zouheir ESSAFI 1007, Tunisie. ' Unite de Proteomie Fonctionnelle and Biopreservation Alimentaire, Institut Superieur des Sciences Biologiques Appliquees de Tunis, Universite Tunis El Manar, Rue Zouheir ESSAFI 1007, Tunisie

Abstract: Lipase enzymes play an important role in lipid metabolism and are produced by a variety of species. Compared with animal, bacterial and fungal, little is known about plant lipases. Although lipases belong to many different protein families, they have the same architecture, the ?/?-hydrolase fold and a conserved active site signature, the Gly-Xaa-Ser-Xaa-Gly motif. Several studies on enzymatic activity and interfacial activation phenomenon of lipases confirm the presence of consensus sequence and a conserved domain. Lipases can be divided into two main groups: carboxylesterases (EC 3.1.1.1); |true| lipases (EC 3.1.1.3), which differ in several biochemical features, which allow us to develop a database that regroups all |true| lipase proprieties to establish relationship between structure and function. LIPABASE is a centralised resource database, which provides information about |true| lipase from different species. It includes general, taxonomic, physicochemical and molecular data. Access to LIPABASE is free and available at http://www.lipabase-pfba-tun.org.

Keywords: LIPABASE database; true lipase; carboxylesterases; bioinformatics; clustering; GxSxG; conserved active site; lipase enzymes; lipid metabolism.

DOI: 10.1504/IJBRA.2011.043770

International Journal of Bioinformatics Research and Applications, 2011 Vol.7 No.4, pp.390 - 401

Available online: 14 Nov 2011

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