Title: Complete coding sequence, sequence analysis and transmembrane topology modelling of Trypanosoma brucei rhodesiense Putative Oligosaccharyl transferase (TbOST II)

Authors: Waren N. Baticados; Noboru Inoue; Chihiro Sugimoto; Hideyuki Nagasawa; Abigail M. Baticados

Addresses: Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos, Laguna 4031, Philippines ' National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan. ' Research Center for Zoonosis Control, Hokkaido University N18-W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan. ' National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan. ' Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Banos, Laguna 4031, Philippines

Abstract: The partial nucleotide sequence of putative Trypanosoma brucei rhodesiense oligosaccharyl transferase gene was previously reported. Here, we describe the determination of its full-length nucleotide sequence by Inverse PCR (IPCR), subsequent biological sequence analysis and transmembrane topology modelling. The full-length DNA sequence has an Open Reading Frame (ORF) of 2406 bp and encodes a polypeptide of 801 amino acid residues. Protein and DNA sequence analyses revealed that homologues within the genome of other kinetoplastid and various origins exist. Protein topology analysis predicted that Trypanosoma brucei rhodesiense putative oligosaccharyl transferase clone II (TbOST II) is a transmembrane protein with transmembrane helices in probably an N(cytosol)-C(cytosol) orientation. Data from the GenBank database assembly and sequence analyses in general clearly state that TbOST II is the STT3 subunit of OST in T.b. rhodesiense that necessitates further characterisation and functional studies with RNAi. TbOST II sequence had been deposited in the GenBank (accession number GU245937).

Keywords: Trypanosoma brucei rhodesiense; oligosaccharyl transferase; N-glycosylation; STT3 subunit; inverse PCR; coding sequences; sequence analysis; transmembrane topology modelling; DNA sequencing.

DOI: 10.1504/IJDMB.2011.043035

International Journal of Data Mining and Bioinformatics, 2011 Vol.5 No.5, pp.574 - 592

Received: 22 Sep 2010
Accepted: 05 Dec 2010
Published online: 24 Jan 2015 *

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