Title: B- and T-cell epitope mapping of human sapovirus capsid protein: an immunomics approach

Authors: M. Ruhul Amin, Mohammad S. Siddiqui, Dilruba Ahmed, Firoz Ahmed, Anowar Hossain

Addresses: Clinical Laboratory Services, International Centre for Diarrhoeal Disease Research, 68, Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh. ' CSIRO, P.O. Box 10041, Adelaide BC, SA 5000, Australia. ' Clinical Laboratory Services, International Centre for Diarrhoeal Disease Research, 68, Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh. ' Clinical Laboratory Services, International Centre for Diarrhoeal Disease Research, 68, Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh. ' Clinical Laboratory Services, International Centre for Diarrhoeal Disease Research, 68, Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh

Abstract: Human sapovirus is one of the major causes of viral gastroenteritis. Although the capsid protein (VP1) confers antigenic cross-reactivity, immunity against sapovirus is still unclear. Using immunoinformatics approach, we defined putative T- and B-cell epitopes of VP1 and mapped on to its predicted three-dimensional structure. Identified five putative T-cell epitopes also occupied the putative B-cell epitope region. These putative epitopes were conserved in all existing serotypes. Predicted epitopes can be generated through proteasome cleavage and may be useful in designing peptide-based subunit vaccine to confer both humoral and cell-mediated immunity.

Keywords: human sapovirus; capsid protein; vaccine; homology modelling; epitope mapping; immunomics; viral gastroenteritis; bioinformatics; sapovirus immunity; immunoinformatics.

DOI: 10.1504/IJBRA.2011.041739

International Journal of Bioinformatics Research and Applications, 2011 Vol.7 No.3, pp.287 - 298

Published online: 02 Aug 2011 *

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