Title: Transcriptional expression of H2B, CTP synthase and PLK3 genes in whole blood exposed to 60Co gamma radiation

Authors: Vinay Jain, Birajalaxmi Das, M. Seshadri

Addresses: Low Level Radiation Studies Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. ' Low Level Radiation Studies Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India. ' Low Level Radiation Studies Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Abstract: Ionising radiation induces complex molecular responses in human cells resulting in changes at mRNA and protein expression. Limited data is available on the transcriptional status of functional genes in response to ionising radiation using peripheral blood mononuclear cells (PBMCs). In the present study, transcriptional profiles of Histone 2B, CTP synthase and PLK3 were studied. Blood samples were collected from ten random healthy males with informed consent. Whole blood irradiation was done at four different dose groups (0.3, 0.6, 1.0 and 2.0 Gy) at a dose rate of 0.68 Gy/minute. PBMCs were separated immediately as well as 4 hours post-irradiation. Total RNA was isolated, transcribed to cDNA and real-time quantitative PCR was performed. Our results revealed a dose-dependent significant upregulation at H2B and CTP synthase at 4 hours post-irradiation. At PLK3 significant upregulation was observed at 2.0 Gy (P = 0.007). In conclusion, these genes can be used for population monitoring programme.

Keywords: whole blood irradiation; transcriptional profile; RT-q-PCR; H2B; CTP synthase; PLK3; low radiation; ionising radiation; peripheral blood mononuclear cells; PBMCs; population monitoring.

DOI: 10.1504/IJLR.2011.040649

International Journal of Low Radiation, 2011 Vol.8 No.1, pp.55 - 65

Available online: 12 Jun 2011 *

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