Title: Predictions of flexible C-terminal tethers of bacterial proteins with the FLEXTAIL bioinformatics pipeline

Authors: Yi Lu, Jeffrey L. Ram

Addresses: Computer Science Department, Prairie View A&M University, Prairie View, TX 77446, USA. ' Department of Physiology, Wayne State University, Detroit, MI 48201, USA

Abstract: Proteins use conserved binding motifs associated with relatively unconserved flexible amino acid sequences as mobile tethers for interacting molecules, as exemplified by C-terminal sequences of bacterial chemotaxis receptors. The FLEXTAIL bioinformatics pipeline predicts flexible tethers and their binding motifs based on the properties of flexibility and sequence conservation. In four groups of bacterial genomes, the algorithm identified >100 putative binding domains, including verifying the known bacterial chemotaxis receptor – NWETF binding motif. Some potential C-terminal flexible regions that have not previously been recognised to function as protein tethers were found and should be investigated further for binding targets and flexibility.

Keywords: bioinformatics pipeline; tether analysis; prediction; bacteria; chemotaxis receptors; bacterial proteins; binding motifs; flexible tethers; protein tethers; binding targets; flexibility.

DOI: 10.1504/IJDMB.2011.039176

International Journal of Data Mining and Bioinformatics, 2011 Vol.5 No.2, pp.174 - 188

Published online: 24 Mar 2011 *

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