Title: Chlorpyrifos-based commercial formulation: alterations in corpuscles of Stannius of catfish

Authors: Ajai Kumar Srivastav, Sanjay Kumar Srivastava, Sarojni Tripathi, Diwakar Mishra, Sunil Kumar Srivastav

Addresses: Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, India. ' Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, India. ' Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, India. ' Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, India. ' Department of Zoology, DDU Gorakhpur University, Gorakhpur 273 009, India

Abstract: Corpuscles of Stannius (CS) and serum calcium of Heteropneustes fossilis were studied after exposure to Chlorpyrifos-Based Commercial Formulation (CBCF) for short-term (1.76 mg L−1 chlorpyrifos) and long-term (0.44 mg L−1 chlorpyrifos) application. The fish were sacrificed after 24, 48, 72 and 96 hours in short-term experiment and after 7, 14, 21 and 28 days in long-term experiment. Hypocalcaemia was noticed in CBCF-treated fish after both short- and long-term exposures. In CBCF-treated H. fossilis, AF-positive cells of CS exhibit increased granulation after 72 and 96 hours, the nuclear volume of these cells remains unchanged. The AF-negative cells of CS of CBCF-treated fish exhibit a slight increase in their nuclear volume after 96 hours. After 21 and 28 days, AF-positive cells of CS show increased granulation. Nuclear volume of these cells exhibited a decrease after 21 and 28 days. A few degenerating cells are also noticed. Nuclear volume of AF-negative cells of CS of CBCF-treated fish increase after 21 and 28 days.

Keywords: corpuscles of Stannius; serum calcium; organophosphate; chlorpyrifos; fish; Heteropneustes fossilis; hypocalcaemia; nuclear volume; cell degeneration.

DOI: 10.1504/IJENVH.2010.037497

International Journal of Environment and Health, 2010 Vol.4 No.4, pp.323 - 332

Published online: 14 Dec 2010 *

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