Title: Ultrastructural alterations induced by tropospheric ozone: comparison between resistant and sensitive clones of Trifolium repens L. CV. Regal

Authors: Adriana Basile, Sergio Esposito, Sergio Sorbo, Elisabetta Napolitano, Barbara Conte, Elisabetta Salvatori, Fausto Manes

Addresses: Department of Biological Sciences, University of Naples Federico II, Napoli, Italy. ' Dipartimento di Biologia Strutturale e Funzionale, University of Naples Federico II, via Cinthia, Napoli, Italy. ' Interdepartmental Service Centre for Electron Microscopy C.I.S.M.E., University of Naples Federico II, via Foria 223, Napoli, Italy. ' Department of Biological Sciences, Section of Plant Biology, University of Naples Federico II, via Foria 223, Napoli, Italy. ' Department of Biological Sciences, Section of Plant Biology, University of Naples Federico II, via Foria 223, Napoli, Italy. ' Department of Plant Biology, Sapienza University of Rome, p.le Aldo Moro, 5 Rome, Italy. ' Department of Plant Biology, Sapienza University of Rome, p.le Aldo Moro, 5 Rome, Italy

Abstract: The effects of ozone on ozone-sensitive (NC-S) and ozone-resistant (NC-R) clones of Trifolium repens L. cv. Regal are compared at ultrastructural level by Transmission Electron Microscopy (TEM). Each clone were exposed to ambient ozone levels in the Botanical Garden of Rome, Italy, according to the ICP Vegetation Experimental Protocol (2005). TEM analyses showed that ultrastructural injuries appeared to be widespread in NC-S leaves. On the contrary, the NC-R clones showed much less damage: the chloroplast maintained an almost intact organisation of thylakoids, but the chloroplast side facing the apoplastic space appeared with no thylakoids. Interestingly, Hsp70 levels, an important stress marker, were only slightly increased in the ozone-sensitive clone with respect to the ozone-resistant clone; in contrast, a strong decrease (−60%) in phosphoenolpyruvate carboxylase (PEPCase) protein levels was measured in the NC-R clone. These data are discussed in the frame of the proposed ozone toxicity mechanism.

Keywords: Trifolium repens; tropospheric ozone; TEM; HSPs; ozone-sensitive; ozone-resistant; Italy; phytotoxic pollutants; air pollution; air quality; biomonitoring; phosphoenolpyruvate carboxylase.

DOI: 10.1504/IJENVH.2010.033713

International Journal of Environment and Health, 2010 Vol.4 No.2/3, pp.260 - 277

Published online: 29 Jun 2010 *

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