Title: Detection of enteroviruses from water samples using optimised RT-PCR with universal primers

Authors: C.M. Zhang, Y.J. Liu, X.C. Wang, X.P. Xue

Addresses: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an, 710055, PR China. ' School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an, 710055, PR China. ' School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an, 710055, PR China. ' Faculty of Life Sciences, Northwestn Polytechnical University, No. 127 Youyi west Road, Xi'an, 710072, PR China

Abstract: A method for rapid detection of enteroviruses in water sample was established by using RT-PCR with universal primers with PV1, PV2, PV3 and CVB3 as the reference viruses. The conditions for simultaneous detection of different enteroviruses were experimentally determined and the detection limit of virus was found to be 38CCID50. Virus concentration, RNA extraction and RT-PCR operation were the main factors affecting the sensitivity of virus detection. By applying the procedures to water samples with seeded PV1 viruses, the interference of impurities in practical waters on enteroviruses detection was investigated. The method was proved to be suitable for practical application.

Keywords: enteroviruses; universal primers; RT-PCR; simultaneous detection; virus concentration; microporous filters; water samples; virus detection; public health; waterborne diseases; reverse transcription polymerase chain reaction.

DOI: 10.1504/IJEP.2009.026658

International Journal of Environment and Pollution, 2009 Vol.38 No.1/2, pp.151 - 165

Available online: 22 Jun 2009 *

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