Title: DNA-protein crosslinks induced by formaldehyde and its repair process

Authors: Wei Zhao, Guangyin Peng, Xu Yang

Addresses: Institute of Pharmacy, Henan University, 475001 Kaifeng, PR China. ' The College of Life Science, Central China Normal University, 430079 Wuhan, PR China. ' The College of Life Science, Central China Normal University, 430079 Wuhan, PR China

Abstract: KCL-SDS assay was used to explore DNA-Protein Crosslinks (DPC) effect induced by formaldehyde (FA) and the repair. Gaseous FA could cause DPC at concentrations as 1.0 mg m−3 and 3.0 mg m−3 (p < 0.01), which was removed within 12 h. Meanwhile, in vitro studies showed that 75 µmol L−1 liquid FA could significantly induce DPC in HepG2 cells, and was removed after 18 h and 24 h. These results suggested that FA could induce DPC at relatively high concentrations, and removing of DPC in vivo required shorter time than that in vitro.

Keywords: formaldehyde; in vivo; in vitro; DPC; DNA-protein crosslinks; repair; DNA; proteins.

DOI: 10.1504/IJEP.2009.025133

International Journal of Environment and Pollution, 2009 Vol.37 No.2/3, pp.299 - 308

Published online: 13 May 2009 *

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