Authors: Jing Zhou, Ren-He Xu, Yong Wang
Addresses: Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA. ' UConn Stem Cell Institute, Department of Genetics and Developmental Biology, University of Connecticut Health Centre, Farmington, CT 06032, USA. ' Department of Chemical, Materials and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
Abstract: Current methods for the extended culture of human embryonic stem cells (hESCs) are far from optimal. Therefore, we proposed to use nanoporous membrane for the co-culture of feeder cells and hESCs. In this proof-of-concept study, we first studied the stability, biocompatibility and permeability of an alginate-poly-L-lysine-alginate membrane. This membrane was then used to encapsulate feeder cells to examine the cell viability and TGF-β secretion. Finally, the encapsulated feeder-conditioned media were used to culture hESCs. The data indicate that nanoporous membrane is a potential tool for the co-culture of feeder cells and hESCs in a safe, efficient, and continuous manner.
Keywords: hESCs; human embryonic stem cells; nanoporous membrane; MEF; mouse embryonic fibroblasts; pathogens; self-renewal; feeder cells; extended culture; stability; biocompatibility; permeability; cell viability.
International Journal of Functional Informatics and Personalised Medicine, 2009 Vol.2 No.1, pp.77 - 88
Available online: 27 Jan 2009 *Full-text access for editors Access for subscribers Purchase this article Comment on this article