Title: Optimisation of cell surface and structural components for improving adsorption capacity of Pseudomonas putida 5-x to Cu2+

Authors: G.H. Zheng, L. Wang, Q. Zhou, F.T. Li

Addresses: School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 200092 Shanghai, China. ' School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 200092 Shanghai, China. ' School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 200092 Shanghai, China. ' School of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 200092 Shanghai, China

Abstract: A heavy metal binding bacterium Pseudomonas putida 5-x was used as biosorbent for heavy metal removal from wastewater. In order to compete with physical adsorbent, the optimisation of the cell preparation technique for further improving its biosorption capacity was conducted. Results showed that the compositions of medium for cell culturing, cell growth age and cell pre-treating technique would affect the cell surface and structural components, hence the adsorption capacity of the cell to heavy metal ions. Using optimal cell culture conditions and pretreatment technique, the Cu2+ adsorption capacity of the cell was obviously increased from 51.2 mg g−1 to 89.6 mg g−1.

Keywords: adsorption capacity; cell culture; cell growth age; cell pretreatment; copper sorption; Pseudomonas putida; biosorption; heavy metals; metal removal; wastewater treatment; water pollution.

DOI: 10.1504/IJEP.2008.020798

International Journal of Environment and Pollution, 2008 Vol.34 No.1/2/3/4, pp.285 - 296

Published online: 16 Oct 2008 *

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