Title: In-silico design of a multi-epitope vaccine candidate against rotaviruses by targeting the outer capsid protein VP-4

Authors: Arijit Das Sharma; Himanshu Singh

Addresses: Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India ' Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India

Abstract: Rotaviral gastroenteritis is a significant public health concern, causing an estimated 145,000 deaths annually in India alone. To develop an effective vaccine, a study was conducted using in-silico and reverse vaccinology approaches. Potent epitopes from Indian neonatal rotaviral strains were identified using IEDB server, and studied for properties such as antigenicity, non-allergenicity, non-toxicity, and interferon-γ inducing abilities. The epitopes were also screened for physiochemical properties using the ProtParam tool. A multi-epitope vaccine was constructed docked with Toll-like Receptor 5 molecule (TLR5) by using ClusPro 2.0 server and was further validated by using in-silico tools. Codon optimisation and in-silico cloning were performed using J-Cat server and SnapGene tool, and finally immune simulation was carried out using C-IMMSIM server.

Keywords: rotavirus; neonatal strain; B and T cell epitopes; human leukocyte antigen; HLA; multi-epitope vaccine; toll like receptors; TLR.

DOI: 10.1504/IJBRA.2024.140040

International Journal of Bioinformatics Research and Applications, 2024 Vol.20 No.3, pp.264 - 286

Received: 10 Sep 2023
Accepted: 02 Jan 2024
Published online: 15 Jul 2024 *

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