Title: Investigation of microbial community structures in typical landfill cover soils of China

Authors: Shangjie Chen; Zhilin Xing; Xia Su; Li Dong; Tiantao Zhao; Juan Li

Addresses: School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China ' School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China ' School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China ' School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China ' School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing, 400054, China ' Chongqing Academy of Chinese Meteria Medica, Chongqing, 400060, China

Abstract: The bacterial community diversity and structure in landfill cover soils which were collected from four typical landfills were characterised by Illumina MiSeq sequencing. The notable differences in physicochemical properties of the four soil samples implied that the soil ecosystem was considerably changeable. Rarefaction results showed that there was high bacterial alpha diversity in landfill cover soils. Principal component analysis (PCA) showed that microbial communities in the different landfill cover soils had significant differences. At phylum level, Proteobacteria represented the dominant phylum, followed by Bacteroidetes, Bacteria_norank, and Chloroflexi. At genera level, the relative abundance of dominant genera were Sphingomonas, Thiobacillus, Oxalobacter and Sandaracinus may play an important role in the soil ecosystem. In addition, correlation analysis indicates that Delta-proteobacteria, Gamma-protebacteria and Sphingobacteria (r = 1.00, P < 0.001) were positively correlated with TN, TP and NO₃^--N. Moreover, a lot of unclassified bacteria suggested a complex microbial community structure in the landfill cover soil.

Keywords: landfill cover soil; MiSeq sequencing; microbial community structure; methanotrophs; soil physicochemical property; environmental factors; dominant genera; methane oxidation capacity.

DOI: 10.1504/IJEP.2022.132939

International Journal of Environment and Pollution, 2022 Vol.71 No.3/4, pp.197 - 211

Received: 09 Jul 2022
Received in revised form: 29 Mar 2023
Accepted: 30 Mar 2023
Published online: 20 Aug 2023 *

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