Title: Rotational and translational alignment errors in 3D reconstruction of virus structures at high resolution

Authors: Yongchang Ji, Dan C. Marinescu

Addresses: School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32816, USA. ' School of Electrical Engineering and Computer Science, University of Central Florida, Orlando, FL 32816, USA

Abstract: The 3D reconstruction of virus structures at high resolution using CryoTEM data requires a very accurate rotational and translational alignment of individual views obtained experimentally. We discuss the geometrical foundations and the computational problems raised by rotational and translational alignment. We also outline the basic ideas for CTF correction.

Keywords: virus structure determination; 3D reconstruction; virus structures; cryo-transmission electron microscopy; cryoTEM; rotational alignment; translational alignment; contrast transfer function; CTF; bioinformatics research; bioinformatics applications; high performance computing.

DOI: 10.1504/IJBRA.2006.011035

International Journal of Bioinformatics Research and Applications, 2006 Vol.2 No.4, pp.359 - 370

Published online: 05 Oct 2006 *

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