Title: Application of bioluminescent bacteria test on evaluating toxicity and monitoring of municipal wastewater

Authors: Zhangyang Song; Hao Wei; Zhaodong Sun; Xiaoyi Sun; Ruifeng Jiao; Yue Wei

Addresses: Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China ' Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China ' Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China ' Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China ' Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China ' Department of Water Resources Assessment, Yellow River Water Resources Protection Institution, Zhengzhou Henan, 450004, China

Abstract: This report describes the application of bioluminescent bacteria test on evaluating toxicity of the inlet and the treated municipal wastewater of the WanShuiQuan wastewater treatment plant. The results found that inlet wastewater was high toxicity, and the 15 min acute toxicity test was consistent with the 30 min test. High toxicity wastewater was largely reduced by the double biological filter bacterial virulence process of wastewater treatment plant. The results showed that a negative correlation was found between the EC₂₀ of inlet wastewater (both15 min and 30 min) and COD_nb/COD, which indicated that the proportion of non-biodegradable chemical oxygen demand (COD_nb) of the total chemical oxygen demand (COD) is higher, the value of EC20 is lower, and the water is more toxic. But there was no apparent linearity between COD_nb/COD and EC₅₀ (both of 15 min and 30 min).

Keywords: acute toxicity; bioluminescent bacteria test; COD_nb/COD; municipal wastewater; Aliivibrio fischeri; hormesis.

DOI: 10.1504/IJEP.2022.132941

International Journal of Environment and Pollution, 2022 Vol.71 No.3/4, pp.229 - 239

Received: 17 Aug 2022
Received in revised form: 22 Apr 2023
Accepted: 27 May 2023
Published online: 20 Aug 2023 *

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