Title: Unusual nucleotide content of Rubella virus genome as a consequence of biased RNA-editing: comparison with Alphaviruses
Author: Vladislav Victorovich Khrustalev, Eugene Victorovich Barkovsky
Department of General Chemistry, Belarussian State Medical University, 83 Dzerzinskogo Prospect, Minsk 220000, Belarus.
Department of General Chemistry, Belarussian State Medical University, 83 Dzerzinskogo Prospect, Minsk 220000, Belarus
Abstract: The usage of cytosine in third codon positions of 22 complete Rubella virus genomes (52.4%) is significantly higher than the usage of guanine (28.9%), adenine (6.9%) and uracil (11.8%). The percentage of U ↔ C transitions (55%) between 22 Rubella virus genomes is two times higher than the percentage of A ↔ G transitions (23%). Predicted microRNA from ORF1 of Rubella virus may target human APOBEC1 mRNA, blocking APOBEC1-editing of viral RNA-minus and RNA-plus strands (preventing G → A and C → U transitions, respectively), while their ADAR-editing (causing U → C and A → G transitions, respectively) occurs frequently.
Keywords: rubella virus genomes; alphavirus; togaviridae; ADAR; APOBEC1; mutational pressure; microRNA; GC-content; inosine; 8-oxo-G; bioinformatics; RNA editing; nucleotide content; nucleotide content.
Int. J. of Bioinformatics Research and Applications, 2011 Vol.7, No.1, pp.82 - 100
Available online: 24 Mar 2011