Title: Kinetics of batch microbial degradation of phenols by indigenous binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens

Authors: Samuel E. Agarry, Thomas O.K. Audu, Bamidele O. Solomon

Addresses: Biochemical Engineering Research Unit, Department of Chemical Engineering, Ladoke Akintola University of Technology, Ogbomoso, Nigeria. ' Department of Chemical Engineering, University of Benin, Benin-City, Nigeria. ' National Biotechnology Development Agency, Abuja, Nigeria; Biochemical Engineering Research Unit, Department of Chemical Engineering, Obafemi Awolowo University, Ile-ife, Nigeria

Abstract: The potential of various organisms to metabolise organic compounds has been observed to be a potentially effective means in disposing of hazardous and toxic wastes. Phenolic compounds have long been recognised as one of the most recalcitrant and persistent organic chemicals in the environment. The bioremediation potential of an indigenous binary mixed culture of Pseudomonas aeruginosa and Pseudomonas fluorescens was studied in batch culture using synthetic phenol in water in the concentration range of 100–500 mg/litre as a model limiting substrate. The effect of initial phenol concentration on the degradation process was investigated. Phenol was completely degraded at different cultivation times for the different initial phenol concentrations. Increasing the initial phenol concentration from 100 mg/litre to 500 mg/litre increased the lag phase from 0 h to 18 h and correspondingly prolonged the degradation process from 24 h to 96 h. There was decrease in biodegradation rate as initial phenol concentration increased. Fitting data into three different kinetic models (Monod, Haldane, and Yano and Koga) showed that the difference in fit between the models was very small and thus statistically insignificant. Thus, the Yano and Koga model has been used to interpret the free cell data on phenol biodegradation. The kinetic parameters have been estimated up to initial phenol concentration of 500 mg/litre. The r_smax decreased, K_s and K_i increased with higher concentration of phenol. The r_smax has been found to be a strong function of initial phenol concentration.

Keywords: binary mixed culture; phenol removal; biodegradation; kinetic modelling; batch cultivation; bioreactors; primary culture; secondary culture; inoculum; bioremediation; kinetics; Pseudomonas aeruginosa; Pseudomonas fluorescens.

DOI: 10.1504/IJEP.2010.035922

International Journal of Environment and Pollution, 2010 Vol.43 No.1/2/3, pp.177 - 189

Published online: 09 Oct 2010 *

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