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Title: |
Multi-layered and conducting polymer-based biosensing membranes |
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Author: |
Yong J. Yuan 
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Address: |
Crown Research Institutes, Industrial Research Ltd., PO Box 31-310, Lower Hutt, New Zealand |
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Journal: |
International Journal of Intelligent Systems Technologies and Applications 2005 - Vol. 1, No.1/2 pp. 126 - 142 |
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Abstract: |
The multi-layer membrane designs of enzyme-based electrode have been proven to be a successful approach for biosensing of formate, with substantial improvement in sensitivity and selectivity. The biological interferents can be eliminated by multi-layer designs and hydrodynamic analysis, prevented from oxidising on the gold substrate. Broad linear ranges of ferrocyanide-mediated formate biosensors have been achieved by discrete layered fabrication. The oxidised enzyme or co-factor can be reduced by a highly flexible redox mediator, which cannot diffuse out of the sensor. The use of multi-layer designs for mediated electron transfer can prevent the mediating species from diffusing away from the electrode surface, when the mediating reaction can take place in a volume, rather than at a surface. A more elaborate trilayer system which consists of mediating, biosensing and diffusion-limiting layers has been developed to eliminate biological interferents. |
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Keywords: |
polypyrrole; multi-layer biosensing membranes; conducting polymer-based biosensing membranes; polymer-based biosensing membranes; formate biosensors; bioengineering; biomechatronics; enzyme-based electrodes; biological interferents. |
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DOI: |
10.1504/IJISTA.2005.007311 |
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