Development of nanoparticle sizing system integrated with optical microscopy using fluorescence polarisation
by Terutake Hayashi; Yasuhiro Takaya; Masaki Michihata
International Journal of Nanomanufacturing (IJNM), Vol. 8, No. 1/2, 2012

Abstract: There is a strong demand for the development of functional nanostructured devices. On the basis of fundamental chemistry, the fields of biotechnology and materials science have developed over the past three decades into powerful disciplines that allow the engineering of advanced technical devices. The functions of nanostructures are greatly influenced by their size and shape. In order to enable precise fabrication of nanostructured devices by bottom-up methods, real-time control based on in situ evaluation of nanoparticle growth and aggregation is needed. The fluorescence polarisation technique allows estimation of the size of a macromolecular structure from rotational diffusion constants, which can be obtained from the correlation of polarisation in fluorescence from fluorophores attached to the macromolecular structure. By analysing the degree of fluorescence polarisation, the theoretical size can be calculated by the Perrin-Weber formula. In this study, we investigated the degree of fluorescence polarisation dependency on different sizes of fluorescein-labelled nanoparticles.

Online publication date: Sun, 01-Jan-2012

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanomanufacturing (IJNM):
Login with your Inderscience username and password:

    Username:        Password:         

Forgotten your password?


Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com