Detoxification of food-waste hydrolysate to enhance lipid production in M. pulcherrima - an alternative feedstock for biodiesel Online publication date: Mon, 13-Aug-2018
by Priyadharshni Vairava Sundaram; Jayanthi Singaram; Thirulogachandar Ashokan
International Journal of Environment and Sustainable Development (IJESD), Vol. 17, No. 2/3, 2018
Abstract: One of the alternative ways to produce biodiesel in a sustainable manner, without contending with food crops, is to use microbes. Microbial oil is a potential feedstock for the biodiesel industry. In this study, oleaginous yeast (Metschnikowia pulcherrima) was used to produce lipid from the carbon source obtained from food waste hydrolysate. Lime and activated charcoal were used to detoxify the hydrolysate and among these detoxifiers, activated charcoal increased the C/N ratio to 76. Fermentation was done in 5 L bioreactor and biomass yield of 12.8 g/L was derived after 96 hours of cultivation. The lipid content was 21.1% and lipid yield was 2.7 g/L with COD removal of 52%. The obtained lipid was analysed using Fourier transform infra-red (FTIR) and the result signifies that the lipid produced using the detoxified food waste hydrolysate could be used as an effective feedstock for biodiesel production.
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